Statistical optimization of L-asparaginase production by E. coli K-12 MTCC 1302

Abstract

L-Asparaginase (L-asparagine amidohydrolase E.C.3.5.1.1) are the enzymes that catalyze the hydrolysis of L-asparagine to L-asparatic acid and ammonia and are clinically important. Objective of the present work was to optimize fermentation conditions for L-asparaginase production by E. coli K-12 (MTCC 1302). The Plackett–Burman experimental design was used to screen selected five independent variables (Lactose, yeast extract, L-asparaginase, CaCl2,and NaCl). The most significant independent variables found to affect enzyme production, namely Lactose, yeast Extract and L-asparagine. These independent variables were further optimized by the central composite design. It was found that optimized L-asparaginase production by E. coli K-12 MTCC 1302 was 3.08 U/mL using Central Composite Design.