Production of Cellulase from Potato, Sapodilla, Kiwi peels and Coir using Trichoderma atroviride ATCC 28043 by Submerged Fermentation

Abstract

Lignocellulosic bioethanol production cost owes close to 40% to the cellulase production processes. A novel combination of potato, Sapodilla, Kiwi peels and coir were used for cellulase production by an optimized submerged fermentation using Trichoderma atroviride ATCC 28043. Submerged Batch fermentation (SMF) were carried out individually using two substrates such as the novel veg-fruit peel, coir substrate and pulverized rice straw to produce cellulases. The activities of cellulase produced in SMF using the novel substrate mixture were FPase 1.66 IU/mL, CMCase 10.85 IU/mL, beta Glucosidases 2705 IU/mL and xylanases 609.9 IU/mL. Using rice straw as the substrate, the enzyme activities were FPase 0.28 IU/mL, CMCase 1.83 IU/mL, beta Glucosidases 456.3 IU/mL and xylanases 102.8 IU/mL. Percentage Saccharification of 43.6% and percentage yield of saccharification of 91.6% were observed for the cellulases produced from the novel substrate mixture.